My research is focused on two small molecules that shape the Earth system and whose roles are profoundly changing due to human activity. Nitrate – the chemical form in which plants assimilate nitrogen – impacts the structure, productivity and biodiversity of ecosystems via a complex cycle of microbially-mediated reactions. Methionine – the initiating amino acid in protein synthesis – is fundamental to all cells and has been a key regulator for the growth of organisms throughout evolution.
Since the beginning of science, improvements in precision measurement have led to discoveries. We are developing a new generation of isotope analytics that is based on ESI-Orbitrap mass spectrometry. This highly accurate technique is opening detailed views into naturally occurring processes. In particular it can reveal the history of a chemical compound, for example the origins of source materials and the process by which they have been combined.
- Unlocking the clumped isotope signatures of marine nitrate
- How much of the methionine in humans is industrially synthesized? - General-purpose stable isotope analyzer (collaboration and consultancy with Thermo Fisher Scientific, Bremen)
IsoX The IsoX software (Isotopolog data eXtraction from Orbitrap RAW files) is freely available upon request from Andreas Hilkert at Thermo Fisher Scientific. Information on how to obtain and use the small tool can be found here. Please email me if you have questions, bug reports or feature requests.
IsoXL (Isotopologs to eXceL) is a web based interface to process and analyze data from IsoX.
Coffinet S, Mühlena L, Lipp JS, Weil M, Neubauer C, Urich T, Hinrichs KU (2021) Evidence for enzymatic backbone methylation of the main membrane lipids in the archaeon Methanomassiliicoccus luminyensis. Applied and Environmental Microbiology [Link]
Neubauer C, Landecker H (2021) A planetary health perspective on synthetic methionine: Implications of opening the tap on a historically limiting nutrient. Lancet Planetary Health [Link]
Hilkert A, Böhlke JK, Mroczkowski SJ, Fort KL, Aizikov K, Wang XT, Kopf SH, Neubauer C (2021) Exploring the potential of Electrospray-Orbitrap for stable isotope analysis using nitrate as a model. Analytical Chemistry [Link]
Neubauer C, Cremiere A, Wang XT, Thiagarajan N, Sessions AL, Adkins JF, Dalleska NF, Turchyn AV, Clegg JA, Moradian A, Sweredoski MJ, Garbis SD, Eiler JM (2020) Stable isotope analysis of intact oxyanions using electrospray Orbitrap mass spectrometry. Analytical Chemistry [Link]
Neubauer C, Sweredoski MJ, Moradian A, Newman DK, Robins RJ, Eiler JM (2018). Scanning the isotopic structure of molecules by tandem mass spectrometry. Int J Mass Spec [Link]
Neubauer C, Kasi AS, Grahl N, Sessions AL, Kopf SH, Kato R, Hogan DA, Newman DK (2018). Refining the application of microbial lipids as tracers of Staphylococcus aureus growth rates in cystic fibrosis sputum. J Bacteriology [Link] [Commentary]
Neubauer C, Sessions AL, Booth IR, Bowen BP, Kopf SH, Newman DK, Dalleska NF (2018). Towards measuring growth rates of pathogens during infections by D2O-labeling lipidomics. Rapid Communications in Mass Spectrometry [Link]
Newman DK, Neubauer C, Ricci JN, Wu C-H, Pearson A (2016). Cellular and molecular biological approaches to interpreting ancient biomarkers. Annu Rev Earth Planet Sci [Link]
Neubauer C, Dalleska NF, Cowley ES, Shikuma NJ, Wu C-H, Sessions AL, Newman DK (2015). Lipid remodeling in Rhodopseudomonas palustris TIE-1 upon loss of hopanoids and hopanoid methylation. Geobiology [Link]
Voorhees RM, Mandal D, Neubauer C, Köhrer C, RajBhandary UL, Ramakrishnan V (2013). The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome. Nat Struct Mol Biol [Link]
Neubauer C, Gillet R, Kelley AC, Ramakrishnan V (2012). Decoding in the absence of a codon by tmRNA and SmpB in the ribosome. Science [Link]
Neubauer C, Gao Y-G, Andersen KR, Dunham CM, Kelley AC, Hentschel J, Gerdes K, Ramakrishnan V, Brodersen DE (2009). The structural basis for mRNA recognition and cleavage by the ribosome-dependent endonuclease RelE. Cell [Link]
Weixlbaumer A, Jin H, Neubauer C, Voorhees RM, Petry S, Kelley AC, Ramakrishnan V (2008). Insights into translational termination from the structure of RF2 bound to the ribosome. Science [Link]
Klages J, Neubauer C, Coles M, Kessler H, Luy B (2005). Structure Refinement of Cyclosporin A in chloroform by using RDCs measured in a stretched PDMS-gel. Chembiochem [Link]
Eiler JM, Neubauer SC, Sweredoski MJ, Griep-Raming J (2018). Isotopic mass spectrometry. Application: WO 2020/035505 A1 [Link]