Understanding factors that shape the Earth's biosphere is a crucial task of our time.
My research is focused on two small molecules that have key ecological functions – nitrate and methionine. Nitrate impacts the structure, productivity and biodiversity of marine and terrestrial ecosystems via a complex cycle of microbially-mediated reactions. Methionine is fundamental to all cells, because it has been a critical regulator for the growth of organisms throughout evolution and up to the present day.
I use isotope ratios to gain insights into naturally occurring processes, mainly by developing a new generation of label-free isotope analytics (isotope-ratio ESI-Orbitrap mass spectrometry). The technique is opening detailed views into the history of biomolecules, in particular the origins of source materials and the pathways by which they have been combined. My hope is that the refined study of structure-process relationships will inform our understanding of the principles that sustain living systems.
I am also interested in how stable isotopes can be used to learn about modern and ancient human societies.
- Unlocking the clumped isotope signatures of marine nitrate
- How much of the methionine in humans is industrially synthesized? - General-purpose stable isotope analyzer (collaboration with Thermo Fisher Scientific, Bremen)
Neubauer C, Landecker H. (in review) Fossil fuel to food: Understanding the synthetic origins of ten percent of human cellular methionine.
Neubauer C, Cremiere A, Wang XT, Thiagarajan N, Sessions AL, Adkins JF, Dalleska NF, Turchyn AV, Clegg JA, Moradian A, Sweredoski MJ, Garbis SD, Eiler JM (2020) Stable isotope analysis of intact oxyanions using electrospray Orbitrap mass spectrometry. Anal Chem [Link]
Neubauer C, Sweredoski MJ, Moradian A, Newman DK, Robins RJ, Eiler JM (2018). Scanning the isotopic structure of molecules by tandem mass spectrometry. Int J Mass Spec [Link]
Neubauer C, Kasi AS, Grahl N, Sessions AL, Kopf SH, Kato R, Hogan DA, Newman DK (2018). Refining the application of microbial lipids as tracers of Staphylococcus aureus growth rates in cystic fibrosis sputum. J Bacteriology [Link] [Commentary]
Neubauer C, Sessions AL, Booth IR, Bowen BP, Kopf SH, Newman DK, Dalleska NF (2018). Towards measuring growth rates of pathogens during infections by D2O-labeling lipidomics. Rapid Commun Mass Spectrom [Link]
Newman DK, Neubauer C, Ricci JN, Wu C-H, Pearson A (2016). Cellular and molecular biological approaches to interpreting ancient biomarkers. Annu Rev Earth Planet Sci [Link]
Neubauer C, Dalleska NF, Cowley ES, Shikuma NJ, Wu C-H, Sessions AL, Newman DK (2015). Lipid remodeling in Rhodopseudomonas palustris TIE-1 upon loss of hopanoids and hopanoid methylation. Geobiology [Link]
Voorhees RM, Mandal D, Neubauer C, Köhrer C, RajBhandary UL, Ramakrishnan V (2013). The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome. Nat Struct Mol Biol [Link]
Neubauer C, Gillet R, Kelley AC, Ramakrishnan V (2012). Decoding in the absence of a codon by tmRNA and SmpB in the ribosome. Science [Link]
Neubauer C, Gao Y-G, Andersen KR, Dunham CM, Kelley AC, Hentschel J, Gerdes K, Ramakrishnan V, Brodersen DE (2009). The structural basis for mRNA recognition and cleavage by the ribosome-dependent endonuclease RelE. Cell [Link]
Weixlbaumer A, Jin H, Neubauer C, Voorhees RM, Petry S, Kelley AC, Ramakrishnan V (2008). Insights into translational termination from the structure of RF2 bound to the ribosome. Science [Link]
Klages J, Neubauer C, Coles M, Kessler H, Luy B (2005). Structure Refinement of Cyclosporin A in chloroform by using RDCs measured in a stretched PDMS-gel. Chembiochem [Link]
Eiler JM, Neubauer SC, Sweredoski MJ, Griep-Raming J (2018). Isotopic mass spectrometry. Application: WO 2020/035505 A1 [Link]